Page 276 - Prevention and Treatment of Incisional Hernia- New Techniques and Materials

Chapter 13
Figure 4. Production of anti-in ammatory cytokines by macrophages seeded on di erent biomaterials in an in ammatory environment (as induced by lipopolysaccharide/interferon γ) compared with a non- stimulated environment after 3 days of culture. a CCL18, b interleukin 1 receptor antagonist (IL-1RA), c RANTES (regulated on activation, normal T cell expressed and secreted), d macrophage-derived chemokine (MDC). The dotted line indicates the basal level of expression, where there is no di erence between stimulated and non-stimulated environments, and the bars denote the mean value. Monocytes from a total of four donors were divided over the di erent biomaterials in triplicate samples. Cells from all donors could not be tested on every biomaterial owing to a low yield of monocytes. Protein production was corrected for DNA before comparison of stimulated and non-stimulated environments. PET+COL, polyethylene terephthalate with a collagen coating; COL, collagen; PET, polyethylene terephthalate; PP, polypropylene. *P<0•050 (Kruskal–Wallis and Mann–Whitney U tests), indicating a signi cant increase in anti-in ammatory cytokines compared with baseline values
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