Page 275 - Prevention and Treatment of Incisional Hernia- New Techniques and Materials

Macrophages in a contaminated environment
Figure 3. Production of proin ammatory cytokines by macrophages seeded on di erent biomaterials in an in ammatory (as induced by lipopolysaccharide/interferonγ) compared with a non-stimulated environment after 3 days of culture. a Tumour necrosis factor (TNF) α, b interleukin (IL) 1β, c monocyte chemotactic protein (MCP)3; d IL-6, e macrophage in ammatory protein (MIP)1α. The dotted line indicates the basal level of expression, where there is no di erence between stimulated and non- stimulated environments, and the bars denote the mean value. Monocytes from a total of four donors were divided over the di erent biomaterials in triplicate samples. Cells from all donors could not be tested on every biomaterial owing to a low yield of monocytes. Protein production was corrected for DNA before comparison of stimulated and non-stimulated environments. PET+COL, polyethylene terephthalate with a collagen coating; COL, collagen; PET, polyethylene terephthalate; PP, polypropylene. *P<0•001, †P<0•050 (Kruskal–Wallis and Mann–Whitney U tests), indicating a signi cant increase in proin ammatory cytokines compared with baseline values.
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