Page 278 - Prevention and Treatment of Incisional Hernia- New Techniques and Materials

Chapter 13
To compare the reaction of macrophages on the four di erent biomaterials in an in ammatory environment, the total amount of protein corrected for DNA is shown (Figure 5). The greatest induction of proin ammatory cytokines TNF-α, IL-1β, MCP-3 and MIP-1α was induced by macrophages on PET+COL biomaterial in the in ammatory environment. The lowest induction of proin ammatory and anti-in ammatory cytokine production was seen on the COL biomaterial (Figure 5). Macrophages on PP biomaterial produced signi cantly more CCL18 and MDC than macrophages on other biomaterials, with the exception of MDC on PET+COL biomaterial. Macrophages on PET+COL biomaterial induced a signi cantly higher RANTES production compared with macrophages on COL (Figure 6). Macrophages on PP and COL biomaterial had the lowest M1/ M2 index, whereas macrophages on PET and PET+COL biomaterials had the highest M1/M2 index in the in ammatory environment (Figure 6).
Taking all the samples together, after correction for multiple testing, signi cant correlations with P < 0.050 were found between MCP-3 and MDC (rs = 0.80), IL-6 and IL-1β (rs = 0.59), MIP-1α and MCP-3 (rs = 0.64), MIP-1α and IL-1β (rs = 0.60), TNF-α and IL-1β (rs = 0.59), and MIP-1α and RANTES (rs = 0.72).
Figure 6. Comparison of secretion of anti-in ammatory cytokines by macrophages seeded on di erent biomaterials at the third day of culture with lipopolysaccharide/interferon γ, corrected for DNA. a CCL18, b interleukin 1 receptor antagonist (IL-1RA), c RANTES (regulated on activation, normal T cell expressed and secreted), d macrophage-derived chemokine (MDC); e M1/M2 macrophage index. Monocytes from a total of four donors were divided over the di erent biomaterials in triplicate samples. Cells from all donors could not be tested on every biomaterial owing to a low yield of monocytes. The M1/M2 index for each sample was calculated as the percentage of the mean for each cytokine. The mean of M1 cytokines (macrophage in ammatory protein1α, tumour necrosis factorα, monocyte chemotactic protein 3, interleukin (IL) 1β, IL-6) was divided by the mean of M2 cytokines (MDC, RANTES, IL-1RA and CCL18). PET+COL, polyethylene terephthalate with a collagen coating; COL, collagen; PET, polyethylene terephthalate; PP, polypropylene. a †P < 0•050 (PP versus all other biomaterials); c †P < 0•050 (PET+COL versus COL); d *P < 0•001 (PP versus PET), †P < 0•050 (PP versus COL); e *P < 0•001 (PP versus PET+COL and PET), †P<0•050 (COL versus PET+COL and PET) (Kruskal–Wallis and Mann–Whitney U tests).
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