Page 120 - Magnesium-based supports for stem cell therapy of vascular disease - Mónica Echeverry Rendón
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CHAPTER 7
Table 2. Daily measurement of pH in medium from flow experiment with Mg samples
3.3 Sprouting assay
The ability of cells to form networks was evaluated through sprouting assay. Cells treated with extracts from the uncoated Mg showed a complete dead of the cells. Oppositely, no statistically detected difference between cells exposed to HMT and MAN in comparison with control were observed. However for HMT the number of tube like structures was lower than for MAN. Fig.4.
Fig.4 Formation of endothelial sprouts on Matrigel in presence of Mg. Cell sprouting and network for- mation was observed in control samples (A). Any structure was observed in cells treated with c.p Mg (B) and contrary death cells were recognized. Oppositely, cells treated with HMT(C) and MAN (D) were comparable with those obtained in the control.
3.4 Paracellular permeability
The integrity of an endothelial monolayer is an important predictor of endothelial function in blood vessels. ECIS data allows for the assessment of endothelial monolayer integrity by the assessment of transendothelial resistance. Changing the culture medium in controls causes a small fluctuation in resistance, which is rapidly normalized (fig.5).
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