Page 100 - Magnesium-based supports for stem cell therapy of vascular disease - Mónica Echeverry Rendón

CHAPTER 6
Fig.1 Toxicity of different cell types after its exposition to leachable of Mg. The response of ASC (A), HUVEC (B), SMC (C), PK84 (D) and THP-1 (E) to leachable of Mg was similar. After the comparison between all the treatments, only a difference is observed with respect to the uncoated samples. HUVEC and SMC shown be more sensitive to Mg concentrations than ASC, PK84 and THP-1.
Fig.2 ASC on surface-coated materials and controls. After 24h of adhesion and proliferation on resp. tissue culture plastic (CTRL), c.p Mg, NAF, HMT, MAN, NAF-HMT and NAF-MAN-modified surfaces, cytoskeleton was visual- ized with phalloidin (green) and the nuclei with DAPI (blue).
Surface coating of materials does not affect proliferation or apoptosis of adhered ASC.
The formation of monolayers on surface-coated Mg, suggested the cells had proliferated and that apoptosis was suppressed. Immunoblotting analyses of ASC adhered to surface-coated materials revealed similar expression of the proliferation marker PCNA compared to ASC on tissue culture plastic (Fig. 3) irrespective of the type of coating.
98