Page 61 - Effects of radiotherapy and hyperbaric oxygen therapy on oral microcirculation Renee Helmers

HBOT and vascular regeneration in oral mucosal flaps
Assessment of FCD was performed by counting the total number of capillary loops per visual field (area of 0.75 mm2) on a 19-inch Samsung SyncMaster 932mv LCD monitor (Samsung, Seoul, South Korea) with a 1440x900-screen resolution. The mean FCD obtained from the 5 isolated image frames, expressed as the number of capillaries per millimeter squared (cpll/mm2), was used to quantify the course of vascular regeneration at each time point. By calculating the mean from the 5 sampled video frames we averaged FCD to represent the overall wound area.
Statistical analysis 3 Sample size was based on a previously reported intraoral wound healing model
with repeated (functional) capillary density measurements.14 Normal distribution
of all microcirculation and supportive data was confirmed with the D’Agostino
and Pearson omnibus normality test. All parameters (FCD, body weight, and whole blood counts) were analyzed separately. Repeated measures two-way analysis of variance (ANOVA) was used to detect overall time effects and time by group effects. Significant main effects were subsequently analyzed using paired-samples t-test (within group over time) and independent-samples t-tests (between groups) with Bonferroni correction. To adjust for innate microcirculatory biological variations, all microvascular measurements were standardized for ease in data interpretation; mean FCD measurements were normalized with respect to baseline and converted into percentages. A p-value less than 0.05 was considered statistically significant; all data are presented in mean ± SD. Data analyses was performed with GraphPad Prism version 5.0 for Windows (GraphPad Software Inc., La Jolla, California, USA).
RESULTS
In both study groups, postoperative flap recovery was uneventful with no signs of wound dehiscence or infection. The wound model and experimental procedures were well tolerated by all subjects and health did not decline at the cost of malnutrition or dehydration as indicated by the significant increase in body weight by day 21 (p<0.05 vs. baseline; paired-samples t-test) in both groups. There were no significant differences in body weight between the two groups and no significant differences in whole blood counts were detected within or between the two groups. A summary of all body weight and whole blood count data is presented in Table 1.
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