Page 123 - Effects of radiotherapy and hyperbaric oxygen therapy on oral microcirculation Renee Helmers

Table 2. Focus depth during CytoCam microcirculation measurement. Data is presented in means±SD.
Buccal angioarchitecture Gingival angioarchitecture
T0 T4 T24 T0 T4 T24
Focus depth [μm] 136 ± 74 146 ± 70 141 ± 53 130 ± 52 116 ± 57 84 ± 95
Angioarchitecture
Temporal classification of buccal and gingival tissue angioarchitecture is presented in Table 3. Overall, the proportion of class 1 angioarchitecture increases overtime although no significant difference was detected (p>0.05).
Table 3. Classification of angioarchitecture of the buccal mucosa and mandibular gingiva in HNCP; class 1 (only capillary loops), class 2 (both capillary loops and vascular network) and class 3 (vascular network without any loops).
Effects of HBOT on late irradiation injury
Class I Class II Class III
Buccal mucosa Mandibular gingiva
T0 T4 T24 T0 T4 T24
59% 74% 76% 79% 69% 81% 37% 26% 24% 17% 31% 19% 3% 0% 0% 4% 0% 1%
Vessel morphology
Altered vessel morphology associated with IR, i.e. telangiectasias in the form
of distortion or “ballooning” of vessels loops, was observed at all time points
in both buccal and gingival mucosa. Furthermore, disorganization of vessel 6 loops remained present at T4 and T24. Figure 2 illustrates images of these microvascular alterations in IR buccal mucosa. Normal (continuous; MFI=3)
blood flow was present in all observed vessels.
Capillary density and blood vessel diameters
An overview of capillary density and buccal Øbv is presented in Table 4. Mean TCD and FCD was the same in all patients and no difference in MFI was observed. A statistically significant decrease in mean Øbv was found between T0 and T24 (20±4 mm vs. 16±5 mm) (p<0.001) and T4 and T24 (20±6 mm vs. 16±5 mm) (p<0.05). No significant difference was detected in mean FCD of the mandibular gingiva between the time points T0, T4 and T24 (respectively 33±16 cpll/mm2, 46±36 cpll/mm2 and 27±36 cpll/mm2). An overall statistically significant increase in mean buccal mucosa FCD was found overtime (T0: 20±11 cpll/mm2, T4: 24±10 cpll/mm2 and T24: 25±07 cpll/mm2) (p<0.05).
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