Page 134 - Helicobacter pylori and Gastric Cancer: From Tumor microenvironment to Immunotherapy

Carcinoma (SRCC). There was no significant difference between the mean age and sex of patients with and without ulcers and cancer. We concluded that our cohort would enable analysis of the link between H. pylori virulence factors and clinical presentation.
The presence of virulence factors is common in the clinical cohort
The PCR-based amplification showed that the cagE, babA2 and babB positive strains had a prevalence of respectively 55.9%, 71.7%, 61.3% in our cohort, whereas cagA, iceA1 and iceA2 were detected in respectively 70.6%, 42.2%, 13.2% of the patients included in this study (Table 2). Thus, the virulence factors selected for this study are commonly detected in our patient cohort and allow statistical analysis as to their relation to disease manifestation.
Specific H. pylori virulence factors show trends of being positively or negatively associated with patient’s clinical presentation
In our study the frequency of cagE-positive isolates obtained from patients with non-ulcer dyspepsia, peptic ulcer dyspepsia or GC patients was 49.4%, 68.4% and 88.9%, respectively, but this apparent positive association with more severe disease did not reach statistical significance (P = 0.198). The babA2 genotype was detected in 74%, 84.2%, and 0% of isolates from NUD, PUD and GC patients, The percentage of babA2- genotype within GC patients was significantly higher than that of cagA+ genotype (P <0.001).
Also the percentage of babB+ were 65.4% and 63.1% for NUD and PUD cases respective ,but for isolates from patients with GC (0%), this apparent negative association was statically significant (P = 0.005). Conversely the prevalence of the iceA2 allele was observed in NUD patients (13.6%) and in PUD (15.8%) and in GC patients (0%), but No significant association was observed between iceA2 genotype and GC (P = 0.779). Similarly, in this study the distribution of iceA1 and clinical outcome was analyzed statistically and it was observed that the frequency of iceA1- positive isolates in NUD, PUD and GC patients was 35.8% and 42.1% and 100%, respectively. The relation of the presence of iceA1 and the development of cases to gastric cancer was statistically significant (P =0.008).
Chapter 6
Chapter 6
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