Page 133 - Helicobacter pylori and Gastric Cancer: From Tumor microenvironment to Immunotherapy

Table 1: Primer sequences for polymerase chain reaction
Gene
Primer
Nucleotide sequence
TTGAAAACTTCAAGGATAGGATAGAGC GCCTAGCGTAATATCACCATTACCC
Ref
babB -F babB-R
ATGAAAAAAACCCTTTTAC CGAATTGCAAGTGATGGT
AGGGATAACAGGCAAGCTTTTGA CTGCAAAAGA TTGTTTGGCAGA
Statistics analysis
Data were analysed by SPSS version 16. The Fisher's exact test or the Chi-square test was used for analysis of categorical data. A p-value of <0.05 was considered statistically significant.
Results
Description of the patient cohort and isolation of study samples
A total of 109 H. pylori samples were isolated from an initial number of 359 biopsies included in this study (yielding an apparent infection rate of 30.4%), of which 60 (55%) were derived from male patients and 49 (45%) isolates came from female patients (sex ratio F/M: 0.82). The average patients’ age at the time of endoscopy was 39 ±17 years. From the 109 patients with established H. pylori infection, 81 patients had presented with a non-ulcer dyspepsia, nineteen patients with a peptic ulcer dyspepsia and nine patients with GC (Signet Ring Cell
Size (bp)
ureC (glmM)
Hp-F HP-R
GGATAAGCTTTTAGGGGTGTTAGGGG GCTTACTTTCTAACACTAACGCGC
(84)
cagE-F cagE-R
294
(85)
cagE
508
babA2-F babA2-R
CCAAACGAAACAAAAAGCGT GCTTGTGTAAAAGCCGTCGT
(6)
babA2
271
(86)
babB
496
cagA-F cagA-R
(87)
cagA
352
iceA1-F iceA1-R
GTGTTTTTAACCAAAGTATC CTATAGCCASTYTCTTTGCA
(84)
iceA1
247
iceA2-F iceA2-R
GTTGGGTATATCACAATTTAT TTRCCCTATTTTCTAGTAGGT
(84)
iceA2
229
H-pylori pathogenicity factors H.pylori pathogenic factors
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