Page 183 - Microbial methane cycling in a warming world From biosphere to atmosphere Michiel H in t Zandt

abundance at 10°C) and Bacteroidales (8.9% relative abundance at 4°C and 13.0% relative abundance at 10°C).
Table 1. Abundance for the 10 metagenome-assembled genomes (MAGs) with >70% completeness. Taxonomic identity was assessed by the GTDB-Tk toolkit. Completeness was assessed by CheckM. Abundance percentages were obtained by mapping back the quality-filtered reads per sample to the MAGs and by averaging the total read corrected coverage data per DNA extraction method.
Complete- 4°C 10°C 4°C 10°C 4°C 10°C ness Acetate Acetate TMA TMA Control Control (%) (%) (%) (%) (%) (%) (%)
Taxonomy
MAG 1: Pelobacteraceae (Family) 100.0 20.2 20.1 1.9 3.2 8.6 5.0
MAG 2: Bacteroidales (Order)
MAG 3: Peptostreptococcales (Order) MAG 4: Methanosarcinaceae (Family) MAG 5: Methanosarcinaceae (Family) MAG 6: Bacteroidales (Order)
MAG 7: Elusimicrobiales (Order) MAG 8: Elusimicrobiales (Order) MAG 9: Methanosarcinaceae (Family) MAG 10: Anaerolineales (Order)
99.5 3.3 3.0 4.3 1.8 4.7 1.7 99.3 5.2 7.7 3.3 4.4 10.1 2.1 97.2 0.4 0.1 52.2 11.5 0.0 0.0
96.8 0.0 0.0 0.2 96.6 5.1 3.7 1.2 88.8 1.8 0.9 0.4 86.0 0.1 1.6 0.0 84.5 8.6 4.0 0.6 77.6 2.1 2.2 0.5
26.8 0.0 0.0 1.8 3.8 3.6 0.9 5.8 5.4 1.4 4.1 6.3
10.3 0.2 0.1 1.4 3.2 4.0
To obtain more insights into the species-specific changes upon methanogenic substrate amendment and temperature increase, we applied de novo assembly and consensus binning to construct MAGs. Binning resulted in 58 MAGs including five high-quality drafts (>90% completeness, <5% redundancy) and five medium-quality drafts (>70% completeness, <10% redundancy) (Supplementary Table S3) (Bowers et al. 2017). In total 77% of the reads could be aligned to all MAGs, with 59% of the reads aligning to the 10 most complete MAGs (Table 1). Three of the top 10 MAGs were identified as methanogenic Methanosarcinaceae. The sequencing coverage of the MAGs was assessed to obtain insights in their relative abundance under the different nutrient amendment and temperature scenarios.
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