Page 155 - Cellular Imaging in Regenerative Medicine, Cancer and Osteoarthritis

SPECT imaging of pro-inflammatory macrophages
Figure 3 Binding of [111In]In-DOTA-JR11 to human synovial tissue. (A) Autoradiography of a section of an OA synovial explant. Dark spots indicate uptake of [111In]In-DOTA-JR11. (B) H&E staining of IFNγ+TNFα-stimulated synovial tissue of the same sample shown in A with 2 40X magnifications. Segmentation lines indicate relevant areas used for analysis. (C) Binding of [111In]In-DOTA-JR11 to human synovial tissue is shown as NET DLU/mm2 + SD. Samples of synovium derived from 3 different OA patients undergoing total knee resection were incubated with (two right bars) and without IFNɣ/ TNFα (two left bars). To determine specific binding a 1000-times excess of unlabeled DOTA-TATE was added to some samples (solid black bars).
CD64 positive cells, indicating pro-inflammatory macrophages, were present from day 1 onward. The sections were ranked to distinguish low-positivity and high-positivity. The number of CD64-positive macrophages were elevated at day 1 and 3, and slowly diminished during the following weeks. There were statistically differences seen between the ranks at the different time points (p<0.0001). No statistically significant differences were seen in the pattern of CD64+ macrophages presence over time between the sham-operated and DMM knees (Fig. 5).
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