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Towardholographic-guidedsurgery1096theobject’spositionfromthemarkers’positionsThetight-fitexperimentwasconductedtodeterminethenavigationerrorAcustom-mademeasuringboardandV-shapedcubeweredesignedinSolidWorks(v2016DassaultSystemsWalthamMAUSA) and 3Dprinted at Oceanz(OceanzEde TheNetherlands)usingtheselectivelasersinteringprintingtechniqueReflectivesphereswereattachedtothecornersofthemeasuringboardandcouldthereforebetrackedbytheIGSsystemandtheyservedastheIGSreferenceframeforplanningInthismeasuringboard16recessesweremadeinwhichthe3D-printedcubewastightlyfitted(Figure2)Reflectivespheres were attached to track the cube The cube wasplacedinallrecessesandthetrackedpositionofthecubeintherecesswascomparedwiththeplannedpositiononthemeasuringboardWiththeintroductionofARanadditionalobjectwastrackedbythetrackingsystem theHoloLens Trackinganextraobjectmightincrease thenavigationerrorTo determine thispotentialincrease the tight-fit experiment wasperformed twiceonceintheconventionalIGSsetup(tight-fitIGS)andoncewhenthe(tracked)HoloLenswasaddedtothesystem(tight-fitAR)Figure2ThevirtualdesignofthemeasuringboardandV-shapedtrackedcube(orange)ThecubewasvirtuallyalignedwithspecificlocationsonthemeasuringboardInthisfigurethecubesareshownattightfittingpositions(green)andloose-fittingpositions(blue)Loose-FitExperimentIntheloose-fitexperimenttheplannedpositionsofthecubewerenotrestrictedbythedesignofthemeasuringboardThecubewaspositionedat21differentplannedpositionsonthemeasuringboard Theloose-fit