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temperature in the dark. The LDH activity was measured in a Synergy HT® spectrophotometer (BioTek Instruments, Winooski, VT, USA) at 490 nm.
Figure A2. Lack of cytotoxic effect of different culture media before and after PFF on MC3T3-E1 osteoblasts as measured by lactate dehydrogenase activity. (A) Before PFF treatment, LDH activity of MC3T3-E1 osteoblasts in a-MEM containing 2% FBS for 1 h; (B) After PFF treatment, LDH activity of MC3T3-E1 osteoblasts without and with PFF in a-MEM containing 10% FBS for 1 h. NS: no significance. LDH, lactate dehydrogenase; FBS, fetal bovine serum; CON, control; PFF, pulsating fluid flow.
Appendix A3
Chapter 3
Figure A3. Cell morphology of MC3T3-E1 pre-osteoblasts at 4 h post-incubation without loading, following treatment with or without 1 h PFF. (A, C) Static control cells. (B, D) 1 h PFF-treated cells.
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