Page 170 - Physico-Chemical Niche Conditions for Bone Cells

General discussion
budding yeast, and neurons. However, little is known about the interaction between F-actin and mitochondria in bone cells.
Moreover, does mechanical loading affect the behavior and function of other organelles, e.g. endoplasmic reticulum, and vesicles, e.g. exosomes? The function of the endoplasmic reticulum is to transport proteins and regulate ATP import from mitochondria. The endoplasmic reticulum interacts with actin filaments, microtubules, and mitochondria, and connects with the nucleus membrane [40,41]. In this thesis, we demonstrated that mechanical loading affected the cytoskeleton structure, mitochondrial network and nucleus morphology. The structure and function of the endoplasmic reticulum might be also affected. Exosomes, as important transporters of miRNAs, play an important role during bone fracture healing. Exosome containing miR-214-3p might be involved in the osteogenesis and angiogenesis, leading to bone formation [42].
5. Does mechanical loading change the behavior and function of cells seeded on a glass slide coated by RGD-functionalized supported lipid bilayers? Poly-L-lysine and rat tail collagen are usually utilized to coat glass slides for cell adhesion. In this thesis, we demonstrated that RGD-functionalized supported lipid bilayers promote osteoblast adhesion and differentiation. In the future, the function of RGD-functionalized supported lipid bilayers as a coating material to enhance cell adhesion should be investigated under mechanical loading.
6. Can a titanium surface be modified using RGD-functionalized supported lipid bilayers, and does such a modified specimen affect osteogenesis in mesenchymal stem cells or osteoblasts? Strategies of implant surface modification to enhance the implant longevity by improving early bone ongrowth include the application of different coating materials to stimulate bone growth at the implant interface. RGD, as implant coating, significantly increases bone formation, but reduces fibrous tissue formation in vivo [43]. In this thesis, osteoblast adhesion and differentiation were enhanced by RGD-functionalized supported lipid bilayers, which might be used as an implant coating material to improve osseointegration in the fields of orthodontics, orthopedics, and implantology.
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