Page 150 - Physico-Chemical Niche Conditions for Bone Cells

RGD-functionalized supported lipid bilayers modulate pre-osteoblasts
Figure 5 Effect of SLBs without or with RGD on focal adhesion formation in MC3T3-E1 pre-osteoblasts after 17 hr of culture. (a) Typical examples of cells on PLL, SLBs without RGD, and SLBs with increasing concentrations of RGD. Cells were stained for integrin a5 (green), phospho-paxillin (yellow), and nuclei (blue). Bar: 50 μm. (b) Number of phospho-paxillin clusters per cell. Cells on PLL had more phospho- paxillin clusters than cells on SLBs with or without RGD (p < 0.001). The number of phospho-paxillin clusters was higher in cells cultured on SLBs with 0.5 μM RGD then in cells cultured on SLBs with 0.2 μM or 1.0 μM RGD (p < 0.05). n = 4 separate experiments, 30–40 cells/substrate/experiment. (c) Mean phospho-paxillin cluster area per cell. The area of phospho-paxillin clusters was larger in cells cultured on PLL then in cells cultured on SLBs with or without RGD (p < 0.001). The mean area of phospho- paxillin clusters in cells cultured on SLBs without RGD was larger than in cells cultured on SLBs +0.2 μM RGD (p < 0.05). n = 4 separate experiments, 30–40 cells/substrate/experiment. (d) Mean integrin
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