Page 152 - Physico-Chemical Niche Conditions for Bone Cells

RGD-functionalized supported lipid bilayers modulate pre-osteoblasts
Figure 6 Effect of SLBs without or with RGD on proliferation-related and osteogenic gene expression in MC3T3-E1 pre-osteoblasts after 17 hr and 1 week of culture. (a) Gene expression in MC3T3-E1 pre- osteoblasts after 17 hr of culture. Relative mRNA expression of Ki67 and CCND1, as well as RUNX2 was similar in cells cultured on the different substrates. OPN expression was higher in cells cultured on SLBs with 0.2 μM RGD than on PLL (p < 0.05). COL1a1 expression was not different between cells cultured on the different substrates. ALP expression was too low to be determined. (b) Gene expression in MC3T3-E1 pre-osteoblasts after 1 week of culture. Relative mRNA expression of proliferation markers Ki67 and CCND1, as well as osteogenic markers RUNX2, OPN, and ALP were similar in cells cultured on the different substrates. COL1a1 expression was increased in cells cultured on SLBs compared to PLL. n = 5. *p < 0.05; **p < 0.02; CCND1, Cyclin D1; OPN, osteopontin; SLBs, supported lipid bilayers; PLL, poly-L-lysine coated glass; COL1a1, collagen type I a1 chain; ALP, alkaline phosphatase; n.d., not detectable.
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