Page 148 - Physico-Chemical Niche Conditions for Bone Cells

RGD-functionalized supported lipid bilayers modulate pre-osteoblasts
Figure 4 Effect of SLBs without or with RGD on MC3T3-E1 pre-osteoblast morphology after 17 hr of culture. (a) Typical examples of cells on PLL, SLBs without RGD, and SLBs with increasing concentrations of RGD. Cells were stained for integrin a5 (green) and nuclei (blue). Bar: 50 μm. (b) Percentage of cells adhered to PLL, SLBs without or with RGD with a small (<1 ´ 103 μm2), medium (1–2 ´ 103 μm2), or large (>2 ´ 103 μm2) surface area. There was a lower percentage of cells with a small (p < 0.001) or a medium surface area (p < 0.05) and a higher percentage of cells with a large surface area (p < 0.001) on PLL than on SLBs with or without RGD. (c) Percentage of cells adhered to PLL, and to SLBs without or with RGD with small (<4 ´ 103 μm3), medium (4–8 ´ 103 μm3), or large (>8 ´ 103 μm3) cell volume. There was a lower percentage of cells with a small volume on PLL than on SLBs with or without RGD (p < 0.05). (d) Eccentricity, a measure for cell elongation, equals 0 for a circle
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