Page 146 - Physico-Chemical Niche Conditions for Bone Cells

RGD-functionalized supported lipid bilayers modulate pre-osteoblasts
Figure 3 Effect of SLBs without or with RGD on MC3T3-E1 pre-osteoblast cell density after 17 hr of culture. Cell density per cm2 on PLL (control), SLB without RGD and with 0.2, 0.5, and 1.0 μM RGD. The cell density was higher on PLL than on SLBs with and without RGD. n ≥ 9 cell cultures from four experiments, **p <0 .001. PLL, poly-L-lysine coated glass; SLB, supported lipid bilayer.
Cell morphology
To investigate the effect of RGD-functionalized supported lipid bilayers on cellular morphology, parameters for cell size (cell surface area, cell volume) and shape of the surface area (elongation, circularity, and extent) were quantified (Table 2). Cells on SLBs with or without RGD exhibited a 0.57-fold smaller surface area and 0.69-fold lower volume than cells on PLL- coated glass (Table 2; p < 0.05; Figure 4). On PLL-coated glass, there was a 0.65-fold higher percentage of cells with a large surface area (>2000 μm2), and a 0.04-fold lower percentage of cells with a small surface area (<1000 μm2; Figure 4b; p <0.001), as well as a 0.15-fold lower percentage of cells with a small volume (<4000 μm3; Figure 4c; p < 0.05) than on SLBs without or with RGD. Cells on SLBs with 0.5 μM RGD had a 1.3-fold larger surface area than cells on SLBs without RGD or with 1.0 μM RGD (Table 2; p < 0.05). There was a trend toward less cells (0.59-fold) with a small surface area (<1000 μm2) and more cells (1.1-fold) with a large surface area (>2000 μm2) on SLBs with 0.5 μM RGD than on SLBs without RGD (Figure 4b; n.s.).
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