Page 35 - Effects of radiotherapy and hyperbaric oxygen therapy on oral microcirculation Renee Helmers

Hyperoxia-driven microvascular changes
RESULTS
Overall the experiments were uneventful and were performed and completed
within the given experimental time frame. A summary of demographics
with baseline hematological values and experimental procedural times 2 are presented in Table 1. All hematological data were within normal ranges
indicating a good state of health in our research subjects.
The MicroScan setup enabled continuous monitoring with sequential measurements of the same microvascular network in the sublingual mucosa across the full experimental procedure. The MicroScan instrument produced a total of 72 video clips of good quality that did not require additional contrast and focus adjustments. Minimal histogram grey scale post-acquisition tweaking (mean X-axis -0.34±0.20, mean Y-axis 2.50±0.28) was performed for each subject’s dataset to reduce investigator interactions with the vascular analysis software by facilitating maximum vessel recognition. For each experiment dataset the same histogram grey scale setting was maintained and used across all time points.
Table 1. Summary of basic demographic, hematology, and investigational procedure times from 8 SPF NZW rabbits. All datasets are presented as mean±SD. Corresponding normal hematological reference ranges from healthy animals from literature14,19† are provided for comparison.
Demographics:
Age Weight
Hematology:
RBC
Hb
PCV (Hct) MCV WBC
PLT
[×1012/L] 5.7 [mmol/L] 7.5 [L/L] 0.38 [fL] 67.1 [×109/L] 7.1 [×109/L] 242
[weeks] [kg]
± ± ± ± ± ±
16 3.4
0.6
0.9 0.04 2.7 0.8 45
± 1 ± 0.3
Normal ranges:
(3.7-7.5)†
(6.2-9.3)† (0.27-0.48)† (58.0-79.6)† (5.0-16.5)† (112-795)†
5 0 0 5
Procedural times:
Induction & instrumentation Normobaric protocol Hyperbaric protocol
Total experimental time
[min] 20 ± [min] 40 ± [min] 40 ± [min] 120 ±
Hb hemoglobin, Hct hematocrit, MCV mean cell volume, NZW
volume, PLT platelet count, RBC red blood cell count, WBC white blood cell count
New Zealand White, PCV packed cell
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