Page 33 - Effects of radiotherapy and hyperbaric oxygen therapy on oral microcirculation Renee Helmers

Hyperoxia-driven microvascular changes
ascertain good health for participation in the second HB part of the experiments.
During the second part of the investigation, blood gas samples were shuttled
in- and outside the chamber through a small airlock. One of the two remaining investigators stationed outside the HB chamber (DMJM, RH, or SH) performed
all microcirculation and blood gas measurements. 2
Figure 2. Overview of the experimental procedures and corresponding time points. BL baseline, FiO2 fraction of inspired oxygen, msw meters of salt water
Microcirculation analysis
All data stored on DVI tapes were converted into AVI files for offline analysis using Adobe Premier Pro 1.5 (Adobe Systems Incorporated, San Jose, California, USA). Microcirculation video clips of 20 sec were captured from each of the 2-min recordings (9 total) corresponding with measurements for both NB and HB procedures. All video clips used for analysis conformed to the recommended standards outlined by a consensus conference based on microcirculation data acquisition and analysis (De Backer et al., 2007).10 All microcirculation data analysis was randomized and analyzed by the same experienced investigator (DMJM).
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