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Chapter 6176the study was approved by the Medical Ethics Committee Leiden The Hague Delft (NL67766.058.18) and the Ethics Committee of the Medical Faculty of the University of Duisburg-Essen (19-8732-BO).StimuliEight identities (four women) displaying anger, fear, happiness, sadness, and neutral expressions were chosen as a source of stimuli from the FEEDTUM database(Wallhoff et al., 2006), which includes videotaped non-instructed reactions to emotion-inducing video-clips, resulting in spontaneous and hence more naturalistic expressions than acted emotional stimuli. The selected videos were standardized so that each clip had a duration of two seconds which included a neutral expression of 500ms followed by 1500ms of each category%u2019s expression, placed in front of a uniform grey background (for the selection and standardization procedure, see Folz et al., 2023). In sum, the stimulus set contained 40 two-second videos, depicting five emotion categories by eight individuals.ProcedureThe general procedure consisted of two separate days. On the first testing day, participants underwent multiple diagnostic assessments and filled in various questionnaires in addition to the questionnaires of interest, which are described in more detail and summarized in the Supplemental Materials in Table S3. The second testing day was scheduled within the following seven days. After being briefed on the procedure, electrodes were attached to the participants%u2019 faces (see Facial Electromyography section) and hands (see Skin Conductance section). Participants were positioned at a distance of 50cm from a Philips screen with a resolution of 1920x1080 pixels. Using E-Prime 3.0 software, the stimuli (720 x 480 pixels) were presented in the center of the screen. The study consisted of two consecutive subtasks. In the first task, participants were instructed to observe the stimuli without engaging in any action while psychophysiological and video data was recorded. Each trial began with the appearance of a black fixation cross on a grey background, lasting for one second, followed by the presentation of one of the 40 video stimuli for two seconds. To mitigate potential data inaccuracies arising from background noise, each participant watched each of the 40 videos twice, resulting in a total of 80 trials. The task had a duration of approximately 20 minutes.