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The research resulting in this thesis has been conducted at the Department of Endodontology, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam the Netherlands; part of the research has been conducted in collaboration with the Department of Dentistry and Oral Health, Aarhus University, Aarhus, Denmark.The research projects that resulted in this thesis were not supported by any funds.Publication of this thesis was supported by Research Institute ACTA, Koninklijke Nederlandse Maatschappij tot bevordering der Tandheelkunde (KNMT) and Nederlandse Vereniging voor Endodontologie (NVvE).Author: Annemarie BaaijDrawings: Annemarie BaaijLay-out and design: Ferdinand van Nispen, my-thesis.nlPrinting: Proefschriftenprinten.nl, Print Service EdeISBN/EAN: 978-90-833601-2-6 © Copyright Annemarie Baaij, 2023All rights reserved. No parts of this publication may be reproduced, stored in a retrieval system or transmitted in any form or by any means without prior written permission of the author.Annemarie Baaij.indd 2 03-08-2023 09:04