Page 27 - Improved endothelialization by silicone surface modification and fluid hydrodynamics modulation- Implications for oxygenator biocompatibility Nasim

proliferation, anti-thrombotic function, as well as decreased platelet adhesion were applied for surface modification of the outside surface of small diameter silicone hollow fibers.
The bioavailability of nitrite, an anti-thrombotic agent, near the surface of
hollow fibers in biohybrid artificial lungs is very important to inhibit platelet 3 adhesion. By applying blood flow circulation in biohybrid artificial lungs, the shear
stress acting on the endothelial cell layer as well as mass transport characteristics
at the fluid-endothelial layer interface will be altered. These changes affect the
transport of nitrite released by the endothelial cell layer and from the nitrite-donor
surface coating. The bioavailability of nitrite depends strongly on diffusion and convection. In Chapter 6, we modeled nitrite bioavailability, expressed as nitrite concentration, on the outside surface of silicone hollow fibers coated with nanoliposomal sodium nitrite-nanoliposomal growth hormone-collagen conjugate
under varying fluid shear stress magnitudes. The nitrite production rate of endothelialized surface-modified silicone hollow fibers was determined experimentally under fluid shear stress in a parallel-plate flow chamber. Laminar
flow and convection-diffusion partial equations in COMSOL were used to simulate
nitrite transport within the parallel-plate flow chamber, and nitrite bioavailability on
the fiber-blood interface. Finally in Chapter 7, we discuss how our results fit with
the hypothesis that silicone surface modification and hydrodynamics modulation
improves endothelialization in order to postulate future directions of research and
clinical application of biohybrid artificial lungs.
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