Page 257 - Prevention and Treatment of Incisional Hernia- New Techniques and Materials

In ammatory response in a contaminated environment
Figure 2. Analysis and ranking for the presence of CD68-positive macrophages 28 and 90 days after implantation of a mesh. An example of CD68-positive cells is shown in which positive cells are indicated by arrows. M indicates mesh. Graphs show the mean rank per type of mesh, p-values are indicated in the graphs, numbers behind the groups indicate sample size.
To investigate how the di erent meshes in uence the macrophage phenotype, we stained the samples with antibodies against iNOS for M1- macrophages and with antibodies against CD206 for M2-macrophages. At day 28 we did not nd signi cant di erences between the conditions, however after 90 days, Parietene Composite® and Sepramesh® were surrounded by signi cantly more iNOS-positive cells than the control group (p=0.03). There were no statistically signi cant di erences between the meshes (Figure 3a). After 28 days we found more CD206-positive cells surrounding C-Qur® and Dualmesh® than in the control group (p=0.045). After 90 days, no signi cant di erences were observed (Figure 3b). To determine for each mesh whether it induces a more pro- or antiin ammatory reaction the M1/M2 ratio was calculated based on iNOS positive and CD206-positive cells (Fig.3c). All meshes except Parietene Composite® after 90 days, had a negative mean ratio, indicative for a predominant M2, or anti-in ammatory, reaction. However no statistically signi cant di erences in M1/M2 ratios were observed between the meshes.
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