Page 256 - Prevention and Treatment of Incisional Hernia- New Techniques and Materials

Chapter 12
Figure 1. A) Analysis of the presence of mast cells at day 28 and day 90 after implantation of a mesh. Graphs show the mean rank per type of mesh, numbers behind the groups indicate sample size. An example of the toluidine staining is shown in which positive cells are indicated by arrows. M indicates mesh. B) Analysis of the presence of T-cells with antibodies against CD3 after 28 and 90 days. An example of the CD3 staining is shown in which positive cells are indicated by arrows. M indicates mesh. Graphs show the mean rank per type of mesh, p-values are indicated in the graphs, numbers behind the groups indicate sample size.
All meshes had more CD3-positive T-cells at day 28 than the control group (p= or < 0.03). There were also mesh-dependent di erences: Parietene® was surrounded by less CD3- positive cells than Dualmesh® (p=0.03) and Parietene Composite® was surrounded by less CD3-positive cells than Omyramesh® (p=0.03). After 90 days still all samples with meshes contained more CD3- positive cells than the control group (p=0.03) (Figure1b). To investigate the total number of attracted macrophages, samples were stained for CD68 as a general macrophage marker. After 28 days more macrophages were found adjacent in the groups with a mesh than in the control group (p= or < 0.015). The same nding was still observed after 90 days, but this was only statistically signi cant for Sepramesh® (p=0.02) and Dualmesh® (p=0.02) (Figure 2).
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