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Plasma microRNAs in oesophageal and gastric cancer935 (a) (b) Figure 2. (a) Cel-miR-39-3p expression levels in all measurable baseline samples (n=52); (b) Cel-miR-39-3p expression levels in all measurable follow-up samples (n=30). Cel-miR-39-3p is shown on the x-axis and the Cq value is shown on the y-axis.Table 2. Patient characteristics with measurable plasma samples. Abbreviations: yrs, years; GC, gastric cancer; EAC, oesophageal adenocarcinoma; ESCC, oesophageal squamous cell carcinoma.Characteristics GCN = 10EACN = 18ESCCN = 8Age (yrs), median (range) 63.1 (50-75) 61 (35-82) 61.8 (52-71)GenderFemaleMale3731535Prior surgery /therapyNoYes 9116271Thermal gradient droplet digital PCRA thermal gradient ddPCR was performed to determine the optimal annealing temperature for each primer assay. For all primer assays 52.7%u00b0C was the most optimal annealing temperature (Supplementary Figure S2) Association of ci-miRNA expression with overall survival and progressionfree survivalGCHigh baseline expression levels of miR-200c-3p showed a trend towards a worse OS (2.8 vs. 11.1 months; HR=2.9 95% CI:0.7-11.8, p=0.06). No significant relation was found between miR-141-3p, miR-146a-5p and miR-218-5p expression and OS or progression-free survival (PFS) in patients with GC (Table 3 and Figure 3a).