Page 48 - Development of Functional Scaffolds for Bone Tissue Engineering Using 3D-Bioprinting of Cells and Biomaterials - Yasaman Zamani

with data by Fu et.al. showing that MC3T3-E1 pre-osteoblast proliferation on graphene oxide/PLGA/hydroxyapatite/bone morphogenic protein-2 (BMP-2) scaffolds stops while osteogenic differentiation follows after 7 days of culture [59]. On the other hand, similar ALP activity and calcium deposition on unmodified controls and 72 h NaOH-treated scaffolds indicate that the surface of the 72 h NaOH-treated scaffolds hampers not only proliferation, but also osteogenic activities. Therefore, 24 h NaOH-treated scaffolds seem more promising in enhancing bone formation.
CONCLUSION
In summary, proliferation, matrix deposition, and differentiation of MC3T3-E1 pre-osteoblasts in 3D-printed PCL scaffolds modified with NaOH treatment or RGD immobilization were investigated in the present study. Our data demonstrate that RGD immobilization (0.011 μg/mg scaffold) on the surface and 24 h NaOH treatment of the surface of 3D-printed PCL scaffold both enhance pre-osteoblast proliferation and matrix deposition while only 24 h NaOH treatment results in increased osteogenic activity, making it the treatment of choice to promote bone formation by osteogenic cells.
ACKNOWLEDGMENTS
The authors wish to extend their thanks to Mr.Kamran Nazmi (Academic Centre for Dentistry Amsterdam, Department of Oral Biochemistry) for synthesis of RGD ligand and valuable inputs. The authors would also like to thank Mr. Arie Werner (Academic Centre for Dentistry Amsterdam, Department of Dental Material Sciences) for help in scanning electron microscopy. The authors declare that there is no conflict of interest.
46