Page 46 - Cellular Imaging in Regenerative Medicine, Cancer and Osteoarthritis
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Chapter 2
5.3. MPIO labeling
Labeling of cells was performed using MPIO (Bangs laboratories, Fisher, IN, USA) at 90% confluence. Different amounts of iron (0, 3.13, 6.25, 12.5, 25, 50 mg which correspond to 0, 2.79 x 106, 5.58 x 106, 1.12 x 107, 2.23 x 107 and 4.47 x 107 particles) were added drop-wise to the cells, which were grown in complete culture medium. For 4, 24 or 48 h the cells were incubated at 37°C/5% CO2. Before further use of labeled cells, the monolayer cultures were rinsed three times with PBS (Invitrogen, Breda, The Netherlands).
Figure 8 Endocytosis dynamics. Hypothesized difference in endocytosis dynamics using different labeling protocols.
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