Page 45 - Cellular Imaging in Regenerative Medicine, Cancer and Osteoarthritis
P. 45
PBS, and incubated with 10% fetal calf serum (Cambrex, Heerhugowaard, The Netherlands) for 1 h, to remove loose SPIO-lipofectamine complexes.
Influence of SPIO cell labeling protocol
B2 80 80
A
60 60 40 40 20 20
00
100 100
% of Max
% of Max
0
1000 2000 3000 FSC-A
4000 0
6.25 ug MPIO 12.5 ug MPIO
1000
2000 3000 4000 SSC-A
25 ug MPIO 50 ug MPIO
0 ug MPIO 3.13 ug MPIO
C
Figure 7 Labeling of C2C12 cells with MPIO. A Forward scatter FACS plots showing no change in cell size following labeling with MPIO in C2C12 cells as opposed to the observation in HUVECs shown in Figure 4. B Side scatter FACS plots showing also increases in cell granularity of C2C12 cells with increasing MPIO incorporation. C Light microscopy image of C2C12 cells in culture after labeling with MPIO at a dose of 25 mg iron/2ml/9.5cm2 for 24 hrs. Note the large amount of MPIO still present outside the cells.
43
