Page 155 - Tailoring Electrospinning Techniques for Regenerative Medicine - Marc Simonet

the main advantage of the additive manufacturing technique is its capability of spatially defined deposition. This enables to plot various cells at a defined and reproducible location in the sca old as for example used in the specific 3D cell clusters and environments provided by Organovo.18
Electrospinning has a strong positon as a stand-alone or additive technique in the field of biofabrication and additive manufacturing. Especially for the regeneration of so and flexible tissue, like a heart valve or blood vessel,
electrospinning might stand the challenge as the only technique used.
With electrospinning, creating submicrometer sized fibers tends to go at the cost of an ordered build-up and a controlled positioned deposition of the fiber. Nevertheless, maintaining a stable electrospinning jet allows to electrospin complex ordered structures at a much lower length scale than most additive manufacturing techniques.
Most known techniques to maintain a stable jet are near field-(Figure 7.8a) and melt electrospinning
GENERAL DISCUSSION
Figure 7.7 Schematic of the fabrication procedure for cell-laden hierarchical sca olds, (a) melt-dispensing for PCL struts, (b) electrospinning 7 process for interlayered micro/nanofibers, and (c) cell-dispensing process for obtaining cell-laden alginate struts. (d) cell-laden hierarchical
sca old and (e) cross-sectional view of the sca old. Reproduced from ref. 32 with permission from The Royal Society of Chemistry.However, a
load bearing bone sca old should better be produced with another biofabrication method.
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