Page 55 - The autoimmune hypothesis of narcolepsy and its unexplored clinical features M.S. Schinkelshoek

Figure 3.4. A. A H1N1-HA275-287 dose-dependent T cell response in a narcolepsy type 1 patient T cell clone (patient number 50, TCC 87) is found only in proliferation experiments performed with irradiated PBMCs that are HLA-DQA1*01:02/DQB1*06:02 (HLA-DQ6)-positive. Experiments were performed in triplicate wells. Significant differences relative to proliferation experiments with irradiated HLA-DQ8 (HLA-DQA1*03:01/DQB1*03:02)-positive PBMCs. B. Anti-HLA-DQ mAb blocks T cell proliferation of the generated TCCs. An anti-HLA-class I mAb blocked T cell proliferation considerably less effectively, whereas anti-HLA-DR and –DP mAbs did not affect T cell proliferation.
* p<0.05, ** p<0.01, *** p<0.001; mAb = monoclonal antibody; PBMCs = peripheral blood mononuclear cells; TCC = T cell clone.
H1N1 reactivity in CD4+ T cells
53
3