Page 35 - The autoimmune hypothesis of narcolepsy and its unexplored clinical features M.S. Schinkelshoek

for HLA-DQB1*06:02 is unchanged between pre- and post-H1N1 NT1 patients with 16.4% in pre-H1N1 NT1 patients and 15.6% in post-H1N1 NT1 patients (p = 0.79; Table 2.3).
Table 2.3. Differences in distribution of HLA-DQB1 alleles in trans with HLA-DQB1*06:02 between pre- and post-H1N1 NT1 patients. P-values are derived from Pearson Chi-Square tests. Pc-values are calculated using the Šidák-Holm correction for multiple comparisons based on k = 13.
HLA associations after 2009 H1N1 pandemic
N
DQ2
DQ4
DQ7
DQ8
DQ9 DQB1*05:01 DQB1*05:02 DQB1*05:03 DQB1*06:01 DQB1*06:02 DQB1*06:03 DQB1*06:04 DQB1*06:09
Pre-H1N1
397
62 (15.6%) 18 (4.5%) 95 (23.9%) 41 (10.3%) 19 (4.8%) 29 (7.3%) 18 (4.5%) 12 (3.0%) 2 (0.5%) 65 (16.4%) 5 (1.3%) 28 (7.1%) 3 (0.8%)
Post-H1N1 OR
128 NA
20 (15.4%) 0.98 6 (4.6%) 1.0 35 (27.3%) 1.2 15 (11.5%) 1.1 2 (1.5%) 0.31 6 (4.6%) 0.61 4 (3.1%) 0.67 6 (4.7%) 1.6 0 (0%) NA 20 (15.4%) 0.93 1 (0.8%) 0.61 13 (10.0%) 1.5 0 (0.0%) NA
95% CI
NA
0.57-1.7 0.40-2.6 0.76-1.9 0.60-2.1 0.07-1.4 0.25-1.5 0.22-2.0 0.58-4.3 NA 0.54-1.6 0.07-5.3 0.74-2.9 NA
p-value pc-value
NA NA
0.949 1 0.969 1 0.436 0.999 0.697 1 0.100 0.747 0.285 0.987 0.520 1 0.368 0.997 0.417 0.999 0.790 1 0.647 1 0.276 0.985 0.320 0.993
H1N1 = 2009 pandemic (H1N1)pdm09 influenza A strain; HLA-DQ2/4/7/8/9 = human leucocyte antigen molecule encoded by the HLA-DQB1*02:01/-DQB1*04:01/-DQB1*03:01 or -03:04/- DQB1*03:02 or -03:05/-DQB1*03:03 allele; NA = not applicable; NT1 = narcolepsy type 1; OR = Mantel-Haenszel common odds ratio; pc-value = corrected p-value.
Multiple HLA-DQ alleles other than HLA-DQB1*06:02 are associated with NT1
In addition to the association between HLA-DQB1*06:02 and NT1, Table 2.2 gives an overview of other HLA-DQ alleles and their association with NT1. HLA-DQ2, HLA-DQB1*05:01 and HLA-DQB1*06:03 are negatively associated with NT1, while HLA-DQ7 and HLA-DQB1*05:02 are more frequently found in NT1 patients than in healthy controls (Figure 2.1).
No differences in HLA-DQB1 alleles in trans with HLA-DQB1*06:02 between pre- and post-H1N1 NT1 patients
HLA-DQ alleles in trans with HLA-DQB1*06:02 are shown for pre- and post- H1N1 NT1 patients separately in Table 2.3. For all alleles in trans with HLA- DQB1*06:02 no difference was shown between patients with symptom onset before the H1N1 pandemic and those with symptom onset after 2009 (Figure 2.1)
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