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Supplementary Figure 2 Effect of the different treatments on fetal astrocyte cell cultures. A: scatterplots of eFluor viability dye staining as analyzed by flow cytometry after different treat- ments. B: quantification of viable cells based on eFluor viability staining. Neither treatment with IL-1β nor rapamycin negatively influenced viability of cell cultures. C: Western blot analysis showed effective reduction of phosphorylated S6 after 24 hours of 100 nM rapamycin treatment. FSC: forward scatter.
