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DYSREGULATION OF THE (IMMUNO)PROTEASOME PATHWAY IN MCD
dolyphosphate p-toluidine salt) was used as chromogenic substrate for AP. Negative control assays were performed without probes (sections were blank).
1. Prabowo AS, van Scheppingen J, Iyer AM, et al. Differential expression and clinical significance of three inflammation-related microRNAs in gangliogliomas. J Neuroinflammation. 2015;12:97.
Supplementary Table 1 Antisense LNA sequences.
+ = LNA modification; m = 2’-O-methyl RNA base; DIG = digoxygenin label.
Supplementary Figure 1 Representative immunoblot analysis of total homogenates from (n=3) surgical hippocampal specimens; β-subunits (β1, ~ 25 kDa; β1i,~ 22 kDa; β5, ~ 25 kDa; β5i, ~ 25 kDa; β-actin ~ 42 kDa).
mRNA
Sequence
Hybridization temperature
β1i (PSMB9)
5’DIG-+TmUmC+CmUmC+CmAmG+TmUmC+TmAmU+CmCmC+A -3’DIG
56°C
β5i (PSMB8)
5’DIG-+AmAmU+CmUmC+AmAmU+CmAmC+CmUmU+GmUmU+C -3’DIG
56°C
105
four