Page 94 - Magnesium-based supports for stem cell therapy of vascular disease - Mónica Echeverry Rendón

CHAPTER 6
Abstract
Magnesium (Mg) is a material that is widely used in industrial applications due its low weight, ductility and good mechanical properties. For non-permanent implants Mg, is a good option because it is biodegradable and its deg- radation products are not harmful for the human body. However, Mg is chemically reactive and hydrogen gas is released as part of the degradation. Using plasma electrolytic oxidation (PEO), a surface layer of MgO/Mg(OH)2 is deposited on the surface of Mg in a controlled way. This way, the degradation rate of the Mg can be reduced in a tun- able manner. In this study, samples of commercial pure of Mg (c.p Mg) were modified by the PEO technique. Vascular cells were more vulnerable than mesenchymal stromal cell from fat (ASC) for compounds released by surface-coated Mg. All cell types supported proliferation of adhered ASC. Released compounds from coated Mg delayed but did not block in vitro wound closure of fibroblast. Preformed endothelial tubes were vulnerable for released compounds, while their supporting ASC were not. As conclusion, it was founded that. Surface-coated of Mg by PEO therefore supports adhesion and future delivery of therapeutic vascular repair cells such as ASC, but the observed vulnerability of vascular cells for coated Mg components needs to be re-assessed in vivo.
Key words: Magnesium, PEO, adipose-derived stromal, ASC, differentiation, cytotoxicity
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