Page 105 - Magnesium-based supports for stem cell therapy of vascular disease - Mónica Echeverry Rendón

PLASMA ELECTROLYTIC OXIDIZED MAGNESIUM ADVERSELY INFLUENCES VASCULAR CELLS TYPES BUT NOT MESENCHYMAL CELLS AND MONOCYTES
Leachables from surface-coated materials do not negatively affect expression of therapeutic genes in ASC. Exposure of leachables from surface-coated materials and from c.p Mg only marginally influenced gene expression of ASC. Expression pro-inflammatory genes IL1B, TNFA and IL6 was low compared to the reference gene GAPDH, while leachables from surface-coated materials had influence. Expression of chemokines genes IL8 and CCL2 was higher and while CCL2 expression was constant, IL8 expression was upregulated more than orders of magnitude by leachables from HMT, NAF-HMT and NAF-MAN. Of the proangiogenic and proregenerative genes, FGF2, IGF1 and HGF were only lowly expressed and not influenced. Of both proangiogenic genes, ANG2 was barely detectable and VEGFA was moderately expressed. VEGFA expression tended to increase marginally upon exposure to leachables. Finally, the highest expression genes by ASC were the basement membrane gene COL4A1 and the fibronectin gene (FN1) the latter was expression tenfold higher than the reference gene. No significant influence of the leachables was detected on expression of both extracellular matrix genes.
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Fig. 8 Gene expression analyses of ASC from three donors exposed to leachables. Expression of genes is expressed as fold-difference (deltaCT) to expression of the reference GAPDH. In general, leachables from resp. NAF, HMT, MAN, NAF-HMT and NAF-MAN surface coatings had little influence on pro-inflammatory genes (IL1B, IL6, IL8, CCL2 and TNFA), pro-regenerative genes (ANG2, VEGFA, FGF2, HGF and IGF1) while the highly expression extracellular matrix genes FN1 and COL4A1 were also unaffected. Only IL8 was highly upregulated after exposure of ASC to HMT, NAF-HMT and NAF-MAN leachables.
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