Page 95 - Physico-Chemical Niche Conditions for Bone Cells

CCCTCAGCAAGCCTGAGAA; Reverse: AGAGGCGTATTAGGAGGCAAG) , runt-related transcription factor-2 (Runx2; Forward: ATGCTTCATTCGCCTCAC; Reverse: ACTGCTTGCAGCCTTAAAT), collagen 1⍺1 (Col1⍺1; Forward: AACTGGTACATCAGCCCGAA; Reverse: TTCCGTACTCGAACGGGAAT ), dentin matrix acidic phosphoglycoprotein-1 (Dmp1; Forward: CGGCTGGTGGACTCTCTAAG; Reverse: CGGGGTCGTCGCTCTGCA TC ), fibroblast growth factor-2 (Fgf2; Forward: GGCTTCTTCCTGCGCATCCA; Reverse: TCCGTGACCGGTAAGTATTG ), matrix extracellular phosphoprotein (Mepe; Forward: GGAGCACTCACTACCTGAC; Reverse: TAGGCACTGCCACCATGT), and osteocalcin (Ocn; Forward: CAGACACCATGAGGACCATCTT; Reverse: GGTCTGATAGCTCGTCACAA). Three to six independent experiments with 24-40 glass slides (Ki67=40, Runx2=40, Col1⍺1=24, Dmp1=38, Fgf2=40, Mepe=36, Ocn=38) (n=3-6; Ki67=3-6, Runx2=3-6, Col1⍺1=3, Dmp1=3-6, Fgf2=3-6, Mepe=3-6, Ocn=3-6) were performed.
Statistical analysis
All data are expressed as mean ± SD from at least three independent, separate experiments. Differences were tested with two-way analysis of variance (ANOVA), combined with Bonferroni. The independent variables were time (post-incubation for 0, 1, 3 and 6 h) and treatment (without and with PFF). Differences were considered significant if p<0.05. Statistical analysis was performed using IBM® SPSS® Statistics version 17.0 software package (SPSS Inc., Chicago, IL, USA) and GraphPad Prism® 5.0 (GraphPad Software Inc., La Jolla, CA, USA).
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