Page 161 - Molecular features of low-grade developmental brain tumours

MIR-519D & MIR-4758 CAN IDENTIFY GG FROM DNT AND ASTROCYTOMAS
Figure 1. Relative expression of miR-519d and miR-4758 in GNTs and astrocytomas. Quantitative real-time PCR. Panels A and B: Relative expression of miR-519d (A) and miR-4758 (B) in GG (n=14), PA (n=15), AII (n=10), AIII (n=14) and GB (n=15). Data are expressed relative to the expression in control cortex (n=7; frozen material). Panels C and D: Expression of miR-519d (C) and miR-4758 (D) in peritumoural GG (n=7), GG (n=14), DNT (n=9) and SEGA (n=10); FFPE. Data are expressed relative to the expression in control cortex (n=9). miRNA expression was normalized to that of the U6B small nuclear RNA gene (Rnu6B) in all cohorts. Dots represent individual samples. The error bars represent SEM. *p < 0.05; **p < 0.01, ***p < 0.001 between experimental samples, Kruskal-Wallis test followed by Mann-Whitney U test; #p < 0.05 between GG and control cortex, Mann-Whitney U test. ctx: control cortex; GG: ganglioglioma; PA: pilocytic astrocytoma; AII: astrocytoma grade II; AIII: astrocytoma grade III; GB: glioblastoma; DNT:
dysembryoplastic neuroepithelial tumour; SEGA: subependymal giant cell astrocytoma.
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