Page 140 - Molecular features of low-grade developmental brain tumours

miRNA gene
miR-320d (p-value; r)
miR-320b (p-value; r)
miR-320c (p-value; r)
miR-625-5p (p-value; r)
miR-330-5p (p-value; r)
miR-3200-3p (p-value; r)
5
CHAPTER 5
Table 4. Correlations of MMPs and TIMPs with miR-320d, miR-320b, miR-320c, miR-625-5p, miR- 330-5p and miR-3200-3p.
MMP2
MMP11
MMP14
MMP15
MMP16 MMP17 MMP19
MMP25 TIMP1
TIMP2 TIMP3 TIMP4
0,010; -0,484
0,016; -0,459
0,002; -0,572
0,013; -0,471
0,074 ;0,349
0,040; 0,397
0,024; -0,433
0,050; 0,380
0,372; -0,179
0,001; -0,590
0,348; -0,188
0,259; -0,225
0,028; -0,423
0,024; -0,432
0,024; -0,433
0,028; -0,422
0,315; 0,201
0,058; 0,369
0,083; -0,340
0,125; 0,302
0,634; -0,096
0,014; -0,467
0,475; -0,143
0,102; -0,321
0,052; -0,379
0,012; -0,477
0,007; -0,503
0,011; -0,481
0,058; 0,37
0,013; 0,47
0,031; -0,416
0,039; 0,399
0,403; -0,168
0,011; -0,483
0,275; -0,218
0,383; -0,175
0,243; 0,271; 0,493; -0,233 -0,22 -0,138
0,03; 0,072 0,062; -0,419 ;-0,351 -0,363
0,019; -0,45
<0.001; -0,627
0,021; 0,443
0,002; 0,56
0,168; -0,273
0,095; 0,328
0,489; -0,139
0,034; -0,41
0,096; -0,327
0,308; -0,204
0,044; -0,391
0,024; -0,433
0,06; 0,367 0,093; 0,33 0,269;
-0,22
0,085; 0,337
0,061; -0,366
<0.001; -0,644
0,004; 0,532 <0.001; 0,633 0,518;
-0,13 0,202; 0,254 0,477;
0,565;
-0,116 -0,143
0,014; 0,084; -0,466 -0,338
0,073; 0,014; -0,351 -0,467
0,251; 0,099; -0,229 -0,324
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Regulation of MMP by miR-320d mimic transfection in fetal astrocytes
To study regulation of MMP2, MMP11, MMP14, MMP15 and MMP19 by miR-320d, fetal astrocytes were transfected with miR-320d mimic. RT-qPCR revealed that transfection with 50 nM miR-320d mimic led to a higher expression of miR-320d (Figure 5a; p<0.05). RNA expression of MMP2, MMP11, MMP14, MMP15 and MMP19 was determined 24 hours after miR-320d transfection using RT-qPCR, and showed a lower expression of MMP2 in miR-320d mimic transfected cells compared to control (Figure 5b; p=0.002), whereas the expression of MMP11, MMP14, MMP15 and MMP19 did not differ between conditions (Figure 5c-f).
Discussion
This study provides evidence that the MMP/TIMP proteolytic system is dysregulated in SEGA. We showed higher expression of MMP2, MMP11, MMP14, MMP15, MMP19, and their endogenous inhibitors TIMP1, TIMP2 and TIMP3 and lower expression of MMP17 in SEGA as compared to periventricular control tissue. Furthermore, we identified the lowly expressed miR-320d in SEGA compared to controls as a potential MMP regulator that can decrease MMP2 expression in vitro.