Page 108 - Molecular features of low-grade developmental brain tumours
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Figure 4. ERK inhibitor U0126 and rapamycin inhibit proliferation of SEGA cells in vitro. a.
Primary SEGA cells from one SEGA-derived cell culture were stimulated for 24 hours with U0126 (5 μM), rapamycin (0.01 μM), a combination of rapamycin with U0126 (rapamycin + U0126) or DMSO (0.05%) as a control. Flow cytometry analysis was used to assay the viability with eFluor or the cell-cycle state using propidium iodide (PI) staining of SEGA cells (n=5). b. Quantification of the PI staining showed lesser cells in the S-phase in the U0126, rapamycin and the rapamycin + U0126 conditions compared to control (0.05% DMSO). Data is expressed relative to the control condition. **p-value<0.01, Kruskal-Wallis test
followed by Mann-Whitney U test.
