Page 26 - Physical activity in recipients of solid organ transplantation - Edwin J. van Adrichem

Chapter 2
547 recipients. Seven recipients with an amputation were excluded.
and research activities are consistent with the Principles of the Declaration of Istanbul as

e Institutional
Review Board approved the study protocol (METc 2001/039), and the reported clinical
outlined in the Declaration of Istanbul on Organ Tra

cking and Transplant Tourism.
Recipient characteristics
23
Physical Activity
A pre-existing database containing information on all RTR treated at the UMCG since
1968 was used to obtain relevant transplant characteristics. CVD history (myocardial
infarction, transient ischemic attack, or cerebrovascular accident) and smoking status were
obtained using a self-report questionnaire. Standard immunosuppressive treatment was
previously described.
27
Measurements
25
per day (MET-min/d). A score of 0 MET-min/day indicates that no notable PA was

e level of PA of the participants during the 12 months prior to the study was estimated
using the validated Tecumseh Occupational Activity Questionnaire (TOAQ) and the
Minnesota Leisure Time Physical Activity Questionnaire (MLTPAQ).
research assistants administered these questionnaires face-to-face.
Consequently, an activity with a MET value of 5 requires
expended at rest. To account for di
performed over resting energy expenditure.

24–26
ve times the energy that is
Trained
e TOAQ measures
frequency, intensity, and duration of three occupational related activities including
commuting.

e MLTPAQ measures leisure time PA including household activities, stair
climbing, and conditioning activities.

e results of both questionnaires were combined by
employing the metabolic equivalents of tasks (MET), a measure of energy expenditure
using the equivalent of the energy expended by an individual while seated at rest.

erences in duration, PA was reported as MET-minutes

e PA assessment has been previously
described in further detail.7
In addition, it was assessed how many RTRs ful

lled the PA
guideline.
According to this guideline, adults should perform 30 minutes of moderate-to-
vigorous PA per day at least

ve days per week, resulting in 150 MET-min/day.
Upon inclusion in the study, blood was drawn after an overnight fasting period (8-12
hours) and before any medication was taken. In these samples, MR-proANP (pmol/l) was
measured with a sandwich immunoassay (MR-proANP LIA; B.R.A.H.M.S). NT-
proBNP (pg/ml) levels were determined by immunoassay on an ELECSYS 2010
instrument (ELECSYS pro-BNP, Roche Diagnostics, Germany). Hs-TnT was measured
on a Roche Modular E170 (Roche Diagnostics, Mannheim, Germany) with a lower
detection limit of 0.003 μg/l.

e estimated glomerular

ltration rate (eGFR) was
calculated with the Chronic Kidney Disease epidemiology collaboration (CKD-EPI)
formula. Creatinine clearance was calculated using 24-hour urinary creatinine excretion
and serum creatinine. 24.