Page 214 - Prevention and Treatment of Incisional Hernia- New Techniques and Materials

Chapter 10
standard rat chow and water ad libitum during the whole study period. The protocol of the study was approved by the Animal Experiments Committee of the Erasmus University Rotterdam.
Materials
Table 1 presents the materials and the brand names of the ve meshes tested. Before use, the biologic sca olds Strattice (Lifecell, KCI, Branchburg, NJ) and Surgisis (Cook Biotech, West Lafayette, IN, USA) were rehydrated or soaked as directed by the manufacturer. Mono lament polypropylene 5-0 (Ethilon; Johnson & Johnson Medical, New Brunswick, NJ, USA) sutures were used for mesh xation to the abdominal wall and closure. Multi lament polyglyconate 5-0 sutures (Sa l; Melsungen, Germany B. Braun) were used for closure of the skin.
Table 1. Meshes included in the study
Brand name
Prolene
Parietex Composite Strattice
Surgisis
Permacol
Procedure
Basic material
Polypropylene
Polyester
Porcine dermis
Small intestinal submucosa
Porcine dermis
Modi cation
None
Collagen-polyethylene- glycol-glycerol coating
Non-crosslinked Non-crosslinked Crosslinked
Manufacturer
Ethicon, Johnson and Johnson (Somerville, NJ, USA)
Covidien Surgical (Dublin, Ireland)
Lifecell, KCI (Branchburg, NJ, USA)
Cook Biotech ( West Lafayette, IN, USA)
Covidien Surgical (Dublin, Ireland)
The study was performed under aseptic conditions using a modi cation of a validated rat model previously described by Burger et al. (14, 22). At the start of the study, the animals were anesthetized using iso urane/O2 inhalation and buprenorphin analgesia (0.05 mg/kg) administered subcutaneously. The abdomen was shaved and cleaned with alcohol 70 %, after which a 5-cm midline skin incision was made and skin aps were raised. Subsequently, the abdominal cavity was opened with a 4-cm midline incision through the linea alba. A sterile mesh measuring 2.5 × 3.5 cm was placed in an intraperitoneal position and xed transmusculary with six nonabsorbable sutures. The abdominal wall and skin were both closed with a running absorbable suture.
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