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(Kits, Klotz and Stein 2015). Our study provides a new method to study interspecies interactions of methane cycle microorganisms under an array of environmental conditions.
Acknowledgements
MitZ, RR, TvdB, MAHJvK and CUW designed, and MitZ, RR, TvdB and MAHJvK performed the experiments. MitZ and RR analyzed the data. MitZ, MSMJ and CUW wrote the article. We thank Guylaine Nuijten for the technical assistance with bioreactor setup and operation and Rob de Graaf for practical help with the acetate determination protocol. We thank Jeroen Frank for technical assistance with genomic data assembly and binning. MitZ, CUW and MSMJ were supported by the Nederlandse Organisatie voor Wetenschappelijk Onderzoek through the Soehngen Institute of Anaerobic Microbiology Gravitation Grant 024.002.002 and the Netherlands Earth System Science Center Gravitation Grant 024.002.001. MAHJvK was supported by the Technology Foundation Stichting voor Technische Wetenschappen Grant 13146. MSMJ was supported by the European Research Council Advanced Grant Ecology of Anaerobic Methane Oxidizing Microbes 339880. The funding agencies had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Supplementary information
Supplementary files can be found at https://static- content.springer.com/esm/art%3A10.1007%2Fs00253-018-9038- x/MediaObjects/253_2018_9038_MOESM1_ESM.pdf or via the QR code.
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