Page 211 - Microbial methane cycling in a warming world From biosphere to atmosphere Michiel H in t Zandt

Figure 4. Fold changes of a) bacteria at class level and b) archaea at family level after long-term warming for the active layer (AL), transition layer (TL), and permafrost (PF). If an assignment to the class level was not available, the closest assignable taxonomic level from the SILVA132 database was used. Non-distinctive taxonomic labels were numbered for clarification. Orange dots indicate an increase upon incubation; blue dots indicate a decrease after incubation. Non-filled orange circles indicate a clade which was below the detection limit in initial samples but present in incubated samples; blue circles indicate the lineage was present in the initial samples but not detected in incubated samples. Bubble sizes represent the fold changes after incubation. Bubble sizes of non-filled circles do not represent fold-changes. Please note the different scales for the bacterial and archaeal plot.
Functional shifts in microbial populations
Overall, the functional profiles at metabolic pathway level showed similar responses and in most cases a decrease upon long-term warming (Fig. 5). The relative gene abundance for CH4 cycling (CH4 production and aerobic oxidation of CH4) showed slight decreases. Similar patterns were also observed for carbon fixation, nitrogen and sulfur cycling genes, despite of the minor differences observed among the different layers.
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