Page 192 - Microbial methane cycling in a warming world From biosphere to atmosphere Michiel H in t Zandt

Chapter 8. Species-specific responses of enriched thermokarst lake sediments
of all Methanosarcinaceae MAGs to the genome of the psychrotolerant methanogen Methanosarcina. lacustris, this genome was used as a reference for the average nucleotide identity (ANI) and average amino acid identity (AAI) calculations (Supplementary Table S4). It has to be noted that the results on the absence of genes have to be interpreted with caution due to the fragmented nature and incompleteness of the MAGs.
Orthologous gene cluster analysis highlighted a large shared fraction between the three Methanosarcinaceae MAGs. A large additional number of gene clusters is shared between MAG 4 and 5. This data is supported by genome-wide average nucleotide identity (ANI) and average amino acid identity (AAI) values (Supplementary Table S4). MAG 4 and MAG 5 are more similar (AAI of 87.8%) and MAG 9 shows higher dissimilarity (AAI of 78.0% to MAG 4 and 78.1% to MAG 5, respectively). Comparison with M. lacustris indicated closest identity of MAG 4 and MAG 5 to M. lacustris (AAI of 88.6% and 86.0%, respectively), whereas MAG 9 was more dissimilar (AAI of 77.4%).
Figure 3. OrthoVenn2 diagram displaying orthologous gene clusters shared between the methanogen Fig. 3
MAGs. The unique numbers in each circle display the singletons and, between brackets, paralogous gene clusters.
190