Page 126 - Development of Functional Scaffolds for Bone Tissue Engineering Using 3D-Bioprinting of Cells and Biomaterials - Yasaman Zamani

PLGA/β-TCP scaffolds post-3D-printing. The findings of this thesis provided new insights on how changes in surface chemistry, topography, mechanical properties, and microenvironment of the cells in the 3D-(bio)printed scaffolds influence pre-osteoblasts responses, which could have important implications for bone tissue engineering in fields such as orthopedics and oral and maxillofacial surgery.
FUTURE PERSPECTIVE
Based on the present thesis, several questions can be raised which deserve attention in the future:
1) Do the surface modifications used on 3D-printed PCL scaffolds in this study have the same
effect on PLGA/β-TCP scaffolds in terms of surface topography?
2) Is mandibular bone tissue growth into the 3D-printed gradient PCL scaffolds different from
tissue ingrowth into the homogeneous scaffolds in vivo?
3) Does incorporation of (natural) bioactive peptides in the alginate bioink formulation improve
osteogenic differentiation potential of the bioink?
4) Does mechanical loading of the 3D-bioprinted PLGA/β-TCP scaffolds containing alginate-
encapsulated pre-osteoblasts improve osteogenic differentiation potential of the 3D-printed
construct?
5) Does dynamic culture of the 3D-bioprinted PLGA/β-TCP scaffolds containing alginate-
encapsulated pre- osteoblasts improve osteogenic differentiation potential of the 3D-printed construct?
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