Page 152 - Fluorescence-guided cancer surgery

150
Chapter 9
of subjects, mean, standard deviation (SD), median, minimum and maximum. Plasma drug concentrations were plotted versus time per individual using both a linear and log y-axis. Additionally, concentration versus time curves were plotted as a spaghetti plot with the median added.
Fluorescent signal in tumor and background was quanti ed using ImageJ (version 1.49b; a public domain, Java-based image processing program developed at the National Institute of Health). Regions of interest (RoI) were drawn with ImageJ on the stored images to quantify uorescent signal in arbitrary units [AU]. Tumor to background ratios (TBRs) were calculated by dividing the uorescent signal of the tumor by uorescent signal of surrounding tissue. To compare TBR and background signal between malignant and benign lesions independent samples t-test was used. TBR was reported in mean, SD, and range.
ACKNOWLEDGEMENTS
We thank: H.A.J.M. Prevoo for her technical assistance in immunohistochemistry staining and uorescence microscopy; dr. G.J. Liefers, dr. J.J. Beltman and prof. dr. J.B.M.Z. Trimbos for their assistance during surgery; M.J.G. Löwik and D.M.A. Berends - van der Meer (Gynecology dept.) and G.M.C. Ranke and E.M.M. Krol - Warmerdam (Surgery dept.) for their assistance during the patient inclusion process.