Page 140 - Cellular Imaging in Regenerative Medicine, Cancer and Osteoarthritis

Chapter 6
4.7. Statistics
Prism software (version 5.01, GraphPad Software) was used for statistical analyses. P values < 0.05 were considered statistically significant. To compare the uptake and internalization of both tracers we used a two tailed student t-test. Curve fitting for kinetic profiling was performed according to the least/ square fit with the Pearson R2 to quantify its goodness of fit.
5. Conclusions
Addition of an albumin binding domain to [177Lu]Lu-DOTA-TATE extended the blood circulation to t1/2= 27.5h. The tumor absorbed dose is raised to 1455 mGy/Bq. Bone marrow of the mouse spine, as dose limiting organ, received 765 mGy/MBq. All other organs also received a high radiation dose, reducing the therapeutic index.
Author Contributions
Conceptualization, TM, BN, MK, YS, MB and MdJ.; methodology, TM, BN; validation, SvT, MK, EdB; formal analysis, SvT, MK; investigation, SvT, EdB; resources, TM, BN, SvT, MK; data curation, SvT; writing—original draft; SvT; writing—review and editing, TM, BN, MK, EdB, YS, MB, MdJ.; visualization, SvT; supervision, MB and MdJ; project administration, SvT.
Acknowledgments
All imaging experiments were conducted at the Applied Molecular Imaging at Erasmus MC Facility (https://www.erasmusmc.nl/en/research/core-facilities/ amie).
Conflicts of Interest
The authors declare no conflict of interest.
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