Page 149 - Tailoring Electrospinning Techniques for Regenerative Medicine - Marc Simonet

Figure 7.2 Averaged (n=5) stress vs. strain curves of original conventional electrospun PLA sca olds compared to LTE spun sca olds original and cultured for 7 days with HVSC. All sca olds consisted of fibers with a 1 mm fiber diameter.
they can be reinforced with layers of a mechanically more robust conventional electrospun structures.
7.2.3 Reproducibility
Since a few years it is getting more commonly accepted that some of the reproducibility challenges of electrospinning are due to the e ects of temperature and relative humidity on the electrospinning process. In the past, without the possibility of climate controlled electrospinning, the electrospinning protocols had to be developed with a large process window in order to be able to
produce fibers at any ambient condition. This was
o en not possible due to solution restrictions like
solubility or desired fiber diameters, especially when
targeting nanometer fibers. Hence no adequate
fibers could be spun, or resulting fibers could exhibit
quite di erent morphologies between batches as
for example shown in Figure 7.3. For both of these 7 sca olds the same spinning protocol was used, but
they were spun at di erent ambient conditions; in the winter, hence dry, respectively summer and hence humid.
GENERAL DISCUSSION
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