Page 59 - The autoimmune hypothesis of narcolepsy and its unexplored clinical features M.S. Schinkelshoek

Conclusions
We identified HLA-DQ6-restricted H1N1-HA peptide-specific T cell responses in a subset of NT1 patients and healthy controls. We did not find HLA-DQ6- H1N1-HA peptide-specific T cells cross-reactive to Hcrt-2 peptides. These results indicate that it is unlikely that cross-reactivity between H1N1-HA and Hcrt-2 peptides presented by HLA-DQ6 is involved in the development of NT1.
Supplementary material Supplementary table 3.1. Amino acid sequences of used peptides.
Protein Fragment
H1N1-HA H1N1-HA275-287 Hcrt-2 Hcrt56-68
Hcrt87-99
Hcrt60-68 (truncated)
Hcrt91-99 (truncated)
Hcrt56-68 (substituted amino acid 1) Hcrt56-68 (substituted amino acid 2)
Amino acid sequence
ERNAGSGIIISDT AGNHAAGILTLGK SGNHAAGILTMGR AAGILTLGK AAGILTMGR AGNuAAGILTLGK AGNAAAGILTLGK
H1N1 reactivity in CD4+ T cells
A = alanine; D = aspartic acid; G = glycine; H = histidine; HA = hemagglutinin; Hcrt = hypocretin; I = isoleucine; K = lysine; L = leucine; M = methionine; N = asparagine; R = arginine; S = serine; T = threonine; u = oxo-histidine.
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